Dihydrofolate reductase (DHFR) Antibody orb215929 from Biorbyt

Product Specs
ItemDihydrofolate reductase (DHFR) Antibody
CompanyBiorbyt
Price Pricing Info Supplier Page
Catalog Numberorb215929
Quantity10 µg, 100 µg
ApplicationsFC, ICC, IF, IHC, WB
Conjugate/TagUnconjugated
FormatLyophilized
ReactivityHuman, Mouse, Rat
TargetDihydrofolate reductase (DHFR)
IsotypeRabbit IgG
HostRabbit
ImmunogenE.coli-derived human DHFR recombinant protein (Position: V2-D187). Human DHFR shares 90% amino acid (aa) sequence identity with both mouse and rat DHFR
NCBI Full Gene Namedihydrofolate reductase
NCBI Gene AliasesDHFR1, DHFRP1, DYR
ClonalityPolyclonal
Molecular Weight21453 MW
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 µg/ml
StorageStore at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles

Specifications/Features

Application Notes: WB: The detection limit for DHFR is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Application Dilutions: Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, Rat, By HeatWestern blot, 0.1-0.5µg/ml, Human, Mouse, RatImmunocytochemistry/Immunofluorescence, 2µg/ml, Human Flow Cytometry, 1-3µg/1x106 cells, Human

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